CALLUS INDUCTION OF GERBERA EXPLANTS WITH DIFFERENT GROWTH REGULATOR COMPOSITIONS

An experiment was accomplished at Advanced Seed Research and Biotech Center (ASRBC), ACI, Dhaka, Bangladesh during the period of September to October, 2014 to evaluate the callus induction of gerbera explants under different growth hormone. Five explants (E₁, Flower bud; E₂, Capitulum; E₃, Leaves; E₄, Peduncle; and E₅, Petiole) and six growth regulator composition T₀: 0 mg/l BAP + 0 mg /l NAA, T₁: 1 mg/l BAP + 0.01 mg /l NAA, T₂:2 mg/l BAP + 0.01 mg /l NAA, T₃: 3 mg/l BAP + 0.01 mg /l NAA, T₄:4 mg/l BAP + 0.01 mg /l NAA, T₅:5 mg/l BAP + 0.01 mg /l NAA were used in this experiment. Individually explant E₁ and treatment T₃ showed best performance in all the studied characteristics. In case of combined effect, T₃E₁ exhibited best performance overall except percent survival where T₂E₁ showed the best result (97.3). So, flower bud can be considered as the most suitable explant for further experimentation in protocol development.