In vitro RAPID REGENERATION OF SAGAR VARIETY OF BANANA

The present study was conducted to find out the effect of benzylaminopurine (BAP) (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L) and indole-3-butyric acid (IBA) (0.5, 1.0, 1.5, 2.0 and 2.5 mg/L) for rapid regeneration of Sagar variety of Banana using shoot tip explants at the Biotechnology Laboratory of Sher-e-Bangla Agricultural University, Dhaka during October 2013 to August 2014. The best response of explants (88%) and highest number of shoots per explants (6.80) were observed with 4.0 mg/L BAP. In contrast, due to combined effect, 4.0 mg/L BAP+2.0 mg/L IBA showed best response (85%) in Sagar. The highest shoot number per explants (5.60) was found with 4.0 mg/L BAP+1.5 mg/L IBA. The highest numbers of roots (5.20 and 5.00 per explants) were observed in 0.5 mg/L of IBA and in combination of 3.0 mg/L BAP+1.0 mg/L IBA. So, individual’s effect of BAP and IBA seemed better than combined effect based on average performance of growth parameters. For acclimatization, plantlets were transplanted from culture media to soil in plastic pots in controlled environment, where the highest survival rate was 95.00% which was almost similar to the survival rate of 94.74% in open atmosphere.